백한우의 성판별 정액을 이용한 수태율 추정에 관한 연구

서지정보

· 발행기관 : 한국수정란이식학회
· 수록지 정보 : Journal of Embryo Transfer / 30권 / 1호
· 저자명 : 김성우, 최진석, 최창용, 김동교, 고응규, 소충실, 성환후

목차

서 론
재료 및 방법
1. 공시축
2. 정액채취와 희석
3. 성판별 정액의 제조
4. 정자의 형태학적 검사
5. 발정동기화, 인공수정 및 임신감정
결 과
1. 백한우 개체의 원 정액 성상과 기형율
2. 백한우 X 정자의 생존성, 정자농도 및 성분리 효율도
3. 백우 성판별 정액의 인공수정 후 수태율과 출산율
고 찰

영어초록

The purpose of this study is to produce wanted sex progeny of genetically confined White Hanwoo (albinism) with preselected sex sperm. One bull of White Hanwoo was chosen for semen donor and X sperm was sorted by MoFlo XDP cell sorter. To compare the pregnancy and birth rates, KPN straw was used as control, total number of unsorted sperm was 20×106/straw. Sexed X frozen semen with 2×106 cells or 4×106 cells per straw were in seminated twice on Hanwoo heifers. The abnormality of the sexed X semen was 24.9 ± 7.31% and distal reflex abnormality of mid piece was significantly (p<0.05) higher (11.7%) compared with that of KPN 768 (5.6%). There were no differences on the pregnancy and birth rates between 2×106 cells or 4×106 cells of X-sperm but KPN semen showed significant differences (p<0.05). The pregnancy rates of KPN 768, 2×106 cells and 4×106 cells X-sperm of White Hanwoo cattle were 85.0%, 26.3% and 50%. The birth rates were 80.0%, 15.8% and 21.4%, respectively. The female offspring rates of KPN 768, 2×106 cells and 4×106 cells X-sperm of White Hanwoo cattle were 43.8%, 100% and 100% (p<0.05). These results indicated that sex sorted White Hanwoo could be used for the production of wanted progeny with 2×106 cells/straw for AI. To increase the efficiency of calf production, the sperm number of sex sorted semen will be optimized for sex selection of White Hanwoo progeny.

참고자료

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