사람 악하선 선암종에서 유도된 SGT 세포 주에서 트랜스글루타민아제 발현

서지정보

· 발행기관 : 대한구강악안면병리학회
· 수록지 정보 : 대한구강악안면병리학회지 / 27권 / 2호
· 저자명 : 이동식, 유종우, 신응섭, 이병헌, 이종헌, 홍성철

목차

I. INTRODUCTION
II. MATERIALS and METHODS
1. Culture conditions of Tumor cell line
2. Morphologic observation
3. Transglutaminase activity assay
4. Immunoslot Blot
III. RESULTS
1. Morphologic features
2. Tranglutaminase activity assay
3. Immunoslot blot
IV. DISCUSSIONS
REFERENCES

영어초록

Established SGT cell line from human submandibular gland adenocarcinoma was used to study the TGase expression on a cellular level in vitro. Transglutaminase 2(TGase 2) is assoacitated with apoptosis, GTP binding protein, and cell marix interaction. The role of TGase 2 in salivary gland tumors is not clear yet. The pupose of this study were to examine the TGase expression of SGT cell line compared to other tumor cell lines, and to apply these results to the pathogenesis of salivary gland tumor. TGase enzyme assay of SGT, SCC-15, HN 4 and HeLa tumor cell line was 3 times repeated, and calculated. Immunoslot blot for semiquantitative protein analysis was done. The obtained results were as follows.
1. SGT cell line showed the highest TGase 2 enzyme activity(about 6-16 folds) irrespective of pre or postconfluency.
2. HN 4 cell line showed the highest TGase 1 enzyme activity(about 2-3 folds) irrespective of pre or postconfluency.
3. Under postconfluency TGase 1 induction was not induced, but slightly increased in all tumor cell lines.
4. TGase enzyme activity in all tumor cell lines was accompanied with TGase protein formation.
From the aboving results, the higher TGase 2 expression of SGT cell line suggested that they would come from submandibular ductal cells and have a important role in the pathogensis of salivary gland tumors.

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