Sox9 regulates development of neural crest and otic placode in a time- and dose-dependent fashion

서지정보

ㆍ발행기관 : 충북대학교 동물의학연구소 ㆍ수록지정보 : Journal of Biomedical Research / 16권 / 1호
ㆍ저자명 : Byung-Yong Park

목차

Introduction
Materials and Methods
Embryo collection and manipulation
Construct
Embryo injections and dexamethasone treatment
Lineage tracing and In situ hybridization
Results
Sox9 is expressed in the neural crest and otic placode at neurula stage
Development of neural crest and otic placode is regulated by Sox9 in time and dose dependent manner
Discussion
Acknowledgements
ORCID
References

영어 초록

Neural crest and placodes share a number of important features, pointing to a possible common evolutionary origin. They both arise from the neural plate border, which is the boundary between the non-neural ectoderm and neural plate. The transcription factor Sox9 has been implicated in neural crest and otic placode induction in several species. To investigate the differential regulation of neural crest and otic placode induction by Sox9, a gain of function assay was performed using a hormone-inducible version of the Sox9 construct at different doses and time periods. Sox9 was expressed in both neural crest and otic placode cell populations in the same stage embryos by in situ hybridization. Using a gain of function approach, increased expression of neural crest marker (Snail2) and otic placode marker (Pax8) in Sox9-overexpressed embryos was observed. Higher dose of Sox9 reduced or eliminated both neural crest and placode cells in the embryos. Interestingly, otic placodes cells were more strongly affected as compared to neural crest cells. So, optimal dosage and timing of Sox9 expression are important for the development of the neural crest and otic placode. The development of the neural crest and otic placode are affected by Sox9 in a time- and dose-dependent manner.

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