BCA assay A+레포트

소개글

BCA assay A+레포트입니다.

목차

1. Title
2. Date
3. Name
4. Purpose
5. Materials
6. Methods
7. Results
8. Discussions
9. Project
10. Reference

본문내용

1. Title
Protein quantification : BCA assay

2. Date

3. Name

4. Purpose
단백질 정량 분석 방법 중 colorimetric method의 일종인 BCA assay를 수행하여 표준 단백질의 흡광도를 이용한 표준곡선을 그린 후, 미지의 단백질 시료 농도를 구해본다.

5. Materials
Extracted proteins (from MCF-7 cells), Bovine serum albumin (BSA) protein, nuclease free water, BCA reagent A & B, micropipette, tip, rack, 96well plate, 1.5㎖ ep-tube, tissue, 70% EtOH, incubator, plate reader

6. Methods
[1] Preparation of standard protein (BSA)
2㎎/㎖ BSA stock (조별로 80㎕씩 제공됨)으로 serial dilution을 진행하여 7개의 ep-tube에 각각 0, 0.025, 0.125, 0.25, 0.5, 1, 2㎎/㎖ 농도의 BSA을 최소 30㎕씩 준비한다.

2㎎/㎖
80㎕(2㎎/㎖ BSA)
30㎕
1㎎/㎖
50㎕(2㎎/㎖ BSA) + 50㎕(D.W)
60㎕
0.5㎎/㎖
40㎕(1㎎/㎖ BSA) + 40㎕(D.W)
40㎕
0.25㎎/㎖
40㎕(0.5㎎/㎖ BSA) + 40㎕(D.W)
40㎕
0.125㎎/㎖
40㎕(0.25㎎/㎖ BSA) + 40㎕(D.W)
60㎕
0.025㎎/㎖
20㎕(0.125㎎/㎖ BSA) + 80㎕(D.W)
100㎕
0㎎/㎖
40㎕(D.W)
40㎕

참고 자료

식품의약품안전처 - 단백질 정량법 매뉴얼
https://www.mfds.go.kr/brd/m_218/view.do?seq=17923&srchFr=&srchTo=&srchWord=&srchTp=&itm_seq_1=0&itm_seq_2=0&multi_itm_seq=0&company_cd=&company_nm=&page=25
(2).https://dawinbio.com/product-information/?q=YToxOntzOjEyOiJrZXl3b3JkX3R5cGUiO3M6MzoiYWxsIjt9&bmode=view&idx=3071998&t=board
Thermo Fisher SCIENTIFIC - BCA Assay and Lowry Assays
https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-assays-analysis/protein-assays/bca-protein-assays.html
Thermo Fisher SCIENTIFIC - Bradford Assay
https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-assays-analysis/protein-assays/bradford-assays.html
BIO RAD - Bradford assay
https://www.bio-rad.com/ko-kr/feature/bradford-assay.html
네이버 지식백과(행정학사전) - 결정계수
https://terms.naver.com/entry.naver?docId=75419&cid=42155&categoryId=42155