Nuclear Remodeling and In Vitro Development Following Somatic Cell Nuclear Transfer in Swine
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 28권 / 4호
ㆍ저자명 : Yoon, Jong-Taek, Kim, Yong-Yeup, Lee, Jong-Wan, Min, Kwan-Sil, Hwang, Seongsoo
ㆍ저자명 : Yoon, Jong-Taek, Kim, Yong-Yeup, Lee, Jong-Wan, Min, Kwan-Sil, Hwang, Seongsoo
목차
INTRODUCTION MATERIALS AND METHODS Oocytes Collection and In Vitro Maturation Treatment for Oocyte Activation Evaluation of Nuclear Status Production of Nuclear Transferred Embryos In Vitro Culture of Embryos Statistical Analysis RESULTS DISCUSSION REFERENCES영어 초록
This study was conducted to investigate nuclear remodeling and developmental rate following nuclear transfer of fetal fibroblast cells, ear skin cells and oviduct epithelial cells into porcine recipient oocytes. To test par-thenogenetic activation, oocytes were treated with a 6-dimethylaminopurine (6-DMAP), a single DC-pulse (DC), calcium ionomycin (ionomycin), DC+6-DMAP and ionomycin + 6-DMAP after in vitro maturation. For nuclear transfer, in vitro matured oocytes were enucleated, and donor cells were transferred into oocytes. Cloned embryos were fused and stimulated with 6-DMAP for 4 h and cultured in vitro for 6 days. Among treatments for parthenogenesis, the activation rate of DC +6-DMAP treatment was significantly higher than that of single treatment roups (p<0.01), except for DC treatment group. However, the difference was not significant in activation rate compared to other complex treatment groups. Nuclear swelling of the cloned embryos was initiated at 60 min after stimulation and increased afterwards. Fusion rates were not different among different donor cells. Cleavage rates of DC treatment groups were significantly higher than those of DC+6-DMAP treatment groups (p<0.05) in case that fetal fibroblast and ear cells were used for nuclear donor. The cloned embryos from developed to blastocysts in oviduct epithelial cell nuclear transfer with DC+6-DMAP treatment was significantly higher compared to those with DC only treatment (p<0.05). However, no blastocyst was developed from nuclear transfer of fetal fibroblast and ear cells regardless of activation treatments. Based on these results, a proper activation stimulation may be necessary to increase the activation rate and the development to blastocyst in cloned porcine embryos.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
- ATP and Purinergic Receptor Agonists Stimulate the Mito..9페이지
- Effects of IVM and IVF Duration on In Vitro Development..6페이지
- Effects of Various Addition and Exclusion Time of Gluco..7페이지
- Effect of Co-Culture with Mammalian Spermatozoa on In V..6페이지
- Derivation of Neural Precursor Cells from Human Embryon..6페이지