Assessment of Sperm Characteristics in Fresh and Frozen Semen of Miniature-Pig
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 28권 / 4호
ㆍ저자명 : Lee S. H., Kim T. S., Cheong H. T., Yang B. K., Kim C. I., Park C. K.
ㆍ저자명 : Lee S. H., Kim T. S., Cheong H. T., Yang B. K., Kim C. I., Park C. K.
목차
INTRODUCTION MATERIALS AND METHODS Semen Collection and Processing Semen Evaluation Freezing and Thawing Statistical Analysis RESULTS AND DISCUSSION ACKNOWLEDGMENTS REFERENCES영어 초록
The present study was conducted to assess sperm characteristics in miniature-pig. The semen samples were transported to the laboratory at 17℃ within 3 hours after collection. The extended semen was stored at 17℃, and sperm quality was evaluated at 0, 1, 3, 5 and 7 days after storage. The semen volume of miniature-pig (62±22㎖) was significantly (p<0.05) lower than that of Duroc (155±25㎖) and Yorkshire (154±23㎖). Significant differences were also observed in sperm concentrations. During 3 days of storage, sperm viability did not differ among miniature-pig, Duroc and Yorkshire. However, the viability was significantly (p<0.05) lower in miniature-pig than in Duroc and Yorkshire semen after Day 3 of storage. In abnormality, acrosome intactness and intensity, there were no differences among miniature-pig, Duroc and Yorkshire semen. On the other hand, the viability of frozen-thawed sperm in miniature-pig was significantly (p<0.05) lower than in that of Duroc and Yorkshire. This study also examined CTC patterns in frozen-thawed spermatozoa. The rates of AR pattern were higher in miniature-pig than in Duroc and Yorkshire. However, no difference was found in F, B and AR patterns. The results of present study suggest that further research is necessary to develop of semen extender and freezing methods to improve sperm quality in miniature-pig.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
- ATP and Purinergic Receptor Agonists Stimulate the Mito..9페이지
- Effects of IVM and IVF Duration on In Vitro Development..6페이지
- Effects of Various Addition and Exclusion Time of Gluco..7페이지
- Effect of Co-Culture with Mammalian Spermatozoa on In V..6페이지
- Nuclear Remodeling and In Vitro Development Following S..5페이지