Guanosine Regulates Germinal Vesicle Breakdown (GVBD) in Mouse Oocytes

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 28권 / 4호
ㆍ저자명 : Cheon Yong-Pil

목차

INTRODUCTION MATERIALS AND METHODS Animals and Oocyte Collection Culture of Oocytes and Chemical Treatments Measurement of cGMP Concentration Statistical Analysis RESULTS Effects of Guanosine on the Denuded Oocyte Maturation Effects of Adenosine on the Denuded Oocyte Maturation Change of [cGMP] during Oocyte Maturation DISCUSSION REFERENCES

영어 초록

Maturation of oocytes is maintained by complex procedures along with follicular genesis and is a critical step for embryonic development. Purine known as an oocyte maturation regulator is present in follicular fluid. In this study, the roles of guanosine as a strong inhibitor of GVBD and a modulator of cyclic GMP concentration in ooyctes were revealed. Denuded immature oocytes were treated with guanosine, and the maturation rates and cGMP concentration of oocytes were measured. GVBD was blocked in a concentration dependent manner by guanosine, but this effect was reversible. However, GVBD was lagged yet not significant by adenosine. Both guanosine and adenosine modified cGMP concentration in oocytes. The characteristic of the guanosine-treated oocyte was significantly higher cGMP compared with the adenosine-treated oocyes at initial time of the maturation. Based these results, guanosine may be a strong and reversible GVBD inhibitor. Although the precise mechanism of guanosine presently is unclear, the results suggest that guanosine may lead the accumulation of cGMP in oocyte cytoplasm, which in turn suppresses GVBD.

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