Polscope-Assisted Enucleation for Nuclear Transfer in Mice
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 28권 / 4호
ㆍ저자명 : Won Ji Young, Kang Jee Hyun, Shim Hosup
ㆍ저자명 : Won Ji Young, Kang Jee Hyun, Shim Hosup
목차
INTRODUCTION MATERIALS AND METHODS Collection of Oocytes Media Preparation of Donor Cells Nuclear Transfer Oocyte Activation RESULTS Induction of Oocyte Activation Somatic Cell Nuclear Transfer DISCUSSION REFERENCES영어 초록
Efficiency of somatic cell nuclear transfer was investigated in mice. First, oocyte activation was induced by SrCl₂, and the rate of development was compared with embryos from normal fertilization. Although more than one half of SrCl₂-treated oocytes developed to blastocysts (146/262, 55.7%), the rate of blastocyst formation was significantly lower than normal fertilization controls (59/79, 74.6%). Second, enucleation of oocytes was performed using Polscope that enables non-invasive visualization of metaphase spindles. Such approach could not only avoid damage of oocytes during an exposure to UV light often employed in conventional enucleation procedures, but could also assure the removal of nuclei from all oocytes operated because of monitoring the location of spindles during an entire process of enucleation. Morphologically normal blastocysts were obtained from the transfer of cumulus cell nuclei into enucleated oocytes. However, the rate of development into the blastocyst stage was still low (4/93, 4.3%). This reflects that the nuclear transfer procedure used in this study was not sufficiently optimized, and other factors may also impact greatly the efficiency of nuclear transfer. Including an induction of oocyte activation and method of enucleation tested in this study, a lot more elements are remained to be optimized to improve the efficiency of somatic cell nuclear transfer in mice.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
- ATP and Purinergic Receptor Agonists Stimulate the Mito..9페이지
- Effects of IVM and IVF Duration on In Vitro Development..6페이지
- Effects of Various Addition and Exclusion Time of Gluco..7페이지
- Effect of Co-Culture with Mammalian Spermatozoa on In V..6페이지
- Nuclear Remodeling and In Vitro Development Following S..5페이지