수산가공식품 중 알레르겐 고등어 혼입여부 신속 검출을 위한 간접효소면역분석법의 개발
(주)코리아스칼라
- 최초 등록일
- 2023.04.05
- 최종 저작일
- 2018.06
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서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 33권 / 3호
ㆍ저자명 : 이정은, 김아윤, 김솔아, 김효인, 박지혜, 심원보
목차
ABSTRACT
Materials and Methods
시료 및 재료
단크론성 항체 생산
간접효소면역분석법(indirect enzyme-linked immunosorbentassay; indirect ELISA)의 개발
수산가공식품 중 고등어 검출
Results and Discussion
단크론성 항체 선택
Indirect ELISA법의 최적화
개발된 indirect ELISA법의 평가
수산 가공 식품 중 고등어 어육 검출
국문요약
References
영어 초록
The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.
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