축수산물에서 LC-MS/MS를 이용한 카라졸롤 및 아자페론 분석

서지정보

ㆍ발행기관 : 한국식품위생안전성학회 ㆍ수록지정보 : 한국식품위생안전성학회지 / 33권 / 3호
ㆍ저자명 : 최수연, 강희승, 김주혜, 천소영, 정지윤, 조병훈, 이강봉

목차

ABSTRACT
Materials and Methods
시약 및 재료
표준원액 및 표준용액의 조제
시료 전처리 방법
시료분석 기기조건
분석법 검정 실험
Results and Discussion
LC-MS/MS를 이용한 분석법 및 최적조건확립
추출 및 정제조건의 최적화
분석방법의 유효성평가
국문요약
References

영어 초록

The aim of the present work was to develop simultaneous methods of quantification of carazolol, azaperone, and azaperol residues in livestock and fishery products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted from beef, pork, chicken, egg, milk and shrimp using acetonitrile (ACN); while flat fish and eel were extracted using 80% ACN. For purification, ACN saturated n-hexane was used to remove fat composition. The standard calibration curves showed good linearity as correlation coefficients; r 2 was > 0.99. Average recoveries expressed were within the range of 67.9-105% for samples fortified at three different levels (0.5 × MRL, 1 × MRL and 2 × MRL). The correlation coefficient expressed as precision was within the range of 0.55- 7.93%. The limit of quantification (LOQ) was 0.0002-0.002 mg/kg. The proposed analytical method showed high accuracy and acceptable sensitivity based on Codex guideline requirements (CAC/GL71-2009). This method can be used to analyze the residue of carazolol, azaperone, and azaperol in livestock and fishery products.

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