Successful In Vitro Development of Preantral Follicles Isolated from Vitrified Mouse Whole Ovaries
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 4호
ㆍ저자명 : Dong-Hoon Kim, Jin-Gu No, Jong-Ju Park, Jin-Ki Park, Jae Gyu Yoo
ㆍ저자명 : Dong-Hoon Kim, Jin-Gu No, Jong-Ju Park, Jin-Ki Park, Jae Gyu Yoo
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Collection of Ovaries Vitrification and Thawing Preantral Follicle Isolation In Vitro Growth (IVG) of Preantral follicles In Vitro Maturation (IVM), Fertilization (IVF) and SubsequentDevelopment Assessment of Cell Number of Blastocyst Statistical Analysis RESULTS Recovery of Preantral Follicles from Vitrified Ovary In Vitro Growth and Maturation of Preantral Follicles Isolated from Vitrified Ovary In Vitro Development of Oocytes within Preantral Follicles DISSCUSSION REFERENCES영어 초록
The purpose of this study was to assess follicular viability and competence through in vitro culture of preantral follicles isolated from vitrified mouse whole ovaries. Mouse preantral follicles were enzymatically isolated from vitrified-warmed and fresh ovaries and cultured for 10 days followed by in vitro oocyte maturation. In vitro matured oocytes were fertilized and cultured to the blastocyst stage. Five minutes pre-exposure to vitrification solution of whole ovaries had significantly higher (p<0.05) oocyte survival and maturation rates than between 10 min exposure groups. Oocyte diameter was significantly smaller (p<0.05) in the 5 and 10 min exposure groups (69.4±2.8 and 67.8±3.1) when compared to that of control group (71.7±2.1). There was no statistical significant difference in blastocyst development rates between vitrification group (8.6%) and the fresh control group (12.0%). The mean number of cells per blastocyst was significantly lower (p<0.05) in the vitrification group (41.9±20.2) than in the fresh control group (55.1±22.5). The results show that mouse oocytes within preantral follicles isolated from the vitrified whole ovaries can achieve full maturation, normal fertilization and embryo development.참고 자료
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