Embryo Gender Ratio and Developmental Potential after Biopsy of In Vivo and In Vitro Produced Hanwoo Embryos
(주)코리아스칼라
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- 2016.04.01
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 4호
ㆍ저자명 : Sang-Rae Cho, In-Cheol Cho, Nam-Young Kim, Sang-Hyun Han, Yong-Sang Park, Moon-Suck Ko, Chang-Young Choe, Jun-Kyu Son, Jae-Gyu Yoo, Hyung-Jong Kim, Yeong-Gyu Ko
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS In Vitro Embryo Production In Vivo Embryo Production Embryo Biopsy LAMP Analysis Experimental Design Statistical Analysis RESULTS DISCUSSION REFERENCES
영어 초록
The present study was to assess the in vitro viability and sexing rate of bovine embryos. Blastocysts were harvested on day 7~9 day after insemination(in vitro and in vivo), and the sex of the embryos was examined using the LAMP method. Embryo cell biopsy was carried out in a 80 μl drop Ca2+, Mg2+ free D-PBS and, biopsied embryos viability were evaluated after more 12 h culture in IVMD culture medium. The formation of recovered embryo to expanded and hatching stages had ensued in higher of sexed embryo in vivo than in vitro (100% vs. 89%, p<0.05), and in vitro, the rates of degeneration after sexing were significantly (p<0.05) higher in vitro than in vivo(11% vs. 0.0%). The rates of the predicted sex were female 61% vs. 56%, and male 39% vs. 44% in vivo and in vitro, respectively. The rates of survival following different biopsy methods were seen between punching and bisection method in vivo and in vitro (100% vs. 89% and 100% vs, 78% respectively). Biopsy method by punching was significantly (p<0.05) higher than bisection between produced embryos in vivo and in vitro. The present data indicate that with microblade after punching for embryo sexing results in high incidence of survivability on development after embryo biopsy. It is also suggested that LAMP-based embryo sexing suitable for field applications.
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