Embryonic Stem Cell-Preconditioned Microenvironment Effects on Epidermoid Carcinoma
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 4호
ㆍ저자명 : Zae Young Ryoo, Myoung Ok Kim
ㆍ저자명 : Zae Young Ryoo, Myoung Ok Kim
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Cell Culture Feeder Preparation and Embryonic Stem (ES) Cell Culture Preparation of 3D-Conditioned Matrix and Reprogramming Induction Cell Viability Test Bisulfite Sequencing RNA Extraction and Real Time RT-PCR 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium Assay Cell Cycle Analysis Telomerase Activity Assay Cellular Aging Assay RESULTS The ES-Preconditioned Microenvironment Induces MorphologicalChanges The ES-Preconditioned Microenvironment Induces Lossof Cancer Cell Properities The ES-Preconditioned Microenvironment Not Induces Cellular Senescence ES-Preconditioned Microenvironment Suppress OncogenicGene Expression DISCUSSION Acknowledgements REFERENCES영어 초록
Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change cell morphology and proliferation. This microenvironment induces cancer cell reprogramming and results in a change in cancer cell properties such as differentiation and migration. The cancer microenvironment affects cancer cell proliferation and growth. However, the mechanism has not been clarified yet. Using the ES-preconditioned 3-D microenvironment model, we provide evidence showing that the ES microenvironment inhibits proliferation and reduces oncogenic gene expression. But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and methylation on Oct4 promoter region. Furthermore, methylation of Nanog was increase on ES-preconditioned microenvironment and supports results that no difference on RNA expression levels. Taken together, these results demonstrated that in the ES-preconditioned 3-D microenvironment is a crucial role for cancer cell proliferation not senescence.참고 자료
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