Genomic Polymorphism Analysis using Microsatellite Markers in Gyeongju Donggyeong Dogs

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 4호
ㆍ저자명 : Seung-Chang Kima, Lee-Kyung Kima, Chang-Min Park, Sun-Ae Park, Yong-Min Cho, Dajeong Lim, Han-Ha Chai, Seung-Hwan Lee, Bong-Hwan Choi, Seog-Kyu Choia, Ji-Woong Lee, Sang-Soo Sun

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Animals and Extraction of Genomic DNA MS Marker for Allele Analysis Multiplex PCR and MS Analysis Statistic analysis RESULTS AND DISCUSSION REFERENCES

영어 초록

This study was conducted to find a useful marker for gene polymorphism analysis using Microsatellite marker (MS marker) in Gyeongju Donggyeong dog. Twenty three MS marker analyzed the genetic features of DNA using 100 Gyeongju Donggyeong dogs in Gyeongju area. It was performed multiplex PCR with 3 set primer divided 9, 10 and 4 by analysis of conditions among MS markers. The results were calculated heterozygosity, polymorphic information content (PIC), allele frequency and number of allele at each locus using Microsatellite Toolkit software and Cervus 3.0 program. Total 148 alleles were genotyped to determine and average 6.43 alleles was detected. FH3381 had the highest of 15 alleles and FH2834 had the lowest of 2 alleles. Expected heterozygosity had a wide range from 0.282 to 0.876 and had average value of 0.6496. Also, Observed heterozygosity had a more wide range from 0.200 to 0.950 and had average value of 0.6404. PIC had range from 0.262 to 0.859 and average PIC was calculated 0.606. Especially, FH2998 represented the highest rate of observed heterozygosity of 0.950 and FH3381 represented the highest rate of expected heterozygosity of 0.876 and PIC of 0.859. The use of these markers was considered to be useful to study genetic traits of Gyeongju Donggyeong dog.

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