담수환경에서 eDNA와 eRNA를 이용한 Microcystin 합성 남조류 탐색 및 세포 내 Microcystin 생합성 활성 변화

서지정보

ㆍ발행기관 : 한국하천호수학회 ㆍ수록지정보 : 생태와 환경 / 56권 / 1호
ㆍ저자명 : 김건희, 박채홍, 조현진, 권대률, 황순진

목차

Abstract
서 론
재료 및 방 법
1. 조사지점 및 시료채집
2. eDNA와 eRNA 추출
3. eDNA와 eRNA의 정량분석 (Quantitative analysis)
4. 남조류 세포밀도 및 Microcystin (MC) 분석
5. 통계분석
결 과
1. 수층에서 mcyA DNA copy number의 시·공간적 변이
2. 수층에서 mcyA 유전자 발현 (RNA gene expression)의시·공간적 변이
3. eRNA발현과 Microcystin (MC) 농도 사이의 관계
고 찰
적 요
REFERENCES

영어 초록

Targeting Microcystin (MC), which is most abundantly detected in the North-Han River water area, we analyzed the relationship between the MC biosynthesis gene (mcyA gene), cyanobacteria cell density, and MC concentration, derived an RNA-MC conversion formula, and derived the cyanobacteria. The concentration of MC present in cells was predicted. In the North-Han River waters, the mcyA gene was found mainly at downstream sites of the North-Han River after Muk-Hyeon Stream junction, and higher copy numbers were found on average than other sites. In the Uiam Lake waters upstream of the North-Han River, the mcyA gene copy number increased at the Kong-Ji Stream point, and after September, the mcyA gene copy number decreased throughout the North-Han River waters. The expression of the mcyA gene was concentrated in the short period of summer due to the spatio-temporal difference between upstream and downstream water bodies. The mcyA gene expression level was not only highly correlated with MC concentration, but also correlated with the cell density of Microcystis aeruginosa and Dolichospermum circinale, which are known to biosynthesize MC. Six conversion formulas derived based on the RNA-MC relationship showed statistical significance (p<0.05) and exhibited high correlation coefficients (r) of 0.9 or higher. The expression level of MC biosynthesis gene present in eRNA determines the synthesis of cyanotoxin substances in water, quickly quantifies gene activity, and can be fully utilized for early warning of MC development.

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