Next-Generation Sequencing을 활용한 어류 위 내용물 분석의 적용 가능성

서지정보

ㆍ발행기관 : 한국하천호수학회 ㆍ수록지정보 : 생태와 환경 / 56권 / 1호
ㆍ저자명 : 박채진, 윤선빈, 이현식, 장서윤, 김강희, 홍동현, 주기재

목차

Abstract
서 론
재료 및 방 법
1. 조사 지점 및 조사 방법
2. 실험 대상 어종
3. 실험 과정
4. 데이터 분석
결 과
고 찰
REFERENCES

영어 초록

The predator-prey interaction in freshwater ecosystems is a crucial area in the ecological study field and one of example to find such interaction is to investigate stomach contents. However, traditional method through visual inspection often induce misidentification, as it depends critically on intactness of physically visible data. In this study, we utilized Next-Generations Sequencing (NGS) technology to test the applicability stomach content analysis and overcome such limitation. NGS was applied to analyze the stomach contents of the Hemibarbus labeo, Tachysurus fulvidraco, and Plecoglossus altivelis collected in the lower part of Nakdong River. As a result, T. fulvidraco had a higher number of Animalia operational taxonomic units (OTUs) intake rate than H. labeo. At the same time, P. altivelis had higher number of Plantae OTUs intake rate than T. fulvidraco and higher Protozoa OTUs intake rate than H. labeo respectively. Therefore, NGS technology application enable to overcome traditional method’s limitation and discover hidden interspecific interaction which can further be used in appropriate habitat assessment.

참고 자료

없음