[A+ 실험레포트] DNA ligation & Bacteria transformation

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"[A+ 실험레포트] DNA ligation & Bacteria transformation"에 대한 내용입니다.

목차

1. Subject: DNA ligation & Bacteria transformation

2. Introduction
I.DNA ligase and ligation
1)DNA ligase and ligation
2)Three main components of a ligation reaction
II.Competent cell and transformation
1)Competent cell
2)Transformation

3. Materials

4. Methods

5. Results

6. Discussion

본문내용

I. DNA ligase and ligation
1. DNA ligase and ligation
Ligation is the joining of two nucleic acid fragment through the action of an enzyme called ligase. The DNA ligase from bacteriophage T4 is the ligase most-commonly used in laboratory research. The mechanism of DNA ligase is to form two covalent phosphodiester bonds between 3' hydroxyl ends of one nucleotide, with 5' phosphate end of another.

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