Taqman PCR
- 최초 등록일
- 2011.06.28
- 최종 저작일
- 2009.10
- 26페이지/ MS 파워포인트
- 가격 1,000원
소개글
TaqMan PCR의 원리 및 장단점, 적용 등에 관한 내용입니다. (영문)
목차
What is real-time PCR
What is TaqMan PCR
Principle of TaqMan PCR
Method of TaqMan PCR
Oligonucleotide probe
Taq DNA polymerase
Advantages and disadvantages of TaqMan PCR
Application of TaqMan PCR
Journal related to TaqMan PCR
References
본문내용
Detection and quantitation of a fluorescent reporter
Quantitates the initial amount of the template most specifically, sensitively and reproducibly
Monitors the fluorescence emitted during the reaction as an indicator of amplicon production during each PCR cycle
(Amplicons are pieces of DNA formed as the products of natural or artificial amplification events)
Offers a much wider dynamic range of up to 107-fold
참고 자료
처음하는 Real time PCR 실험강좌, Life Science & Biotechnology 62~65.
www.enigmadiagnostics.com/template2.php?page=...
www.bio.miami.edu/~cmallery/150/gene/Taq.htm
www.nature.com/.../n12/box/nrg1201-930a_BX2.html
Sambrook and Russell. Molecular cloning a laboratory manual third edition.
Jessica L. Versage, Darlena D. M. Severin, May C. Chu, and Jeannine M. Petersen. Development of a Multitarget Real-Time TaqMan PCR Assay for Enhanced Detection of Francisella tularensis in Complex Specimens. JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2003, p. 5492–5499