Spermatogenic and Antioxidant Potential of Mucuna prureins (L.) in Epididymal Spermatozoa: A Dose Dependent Effect

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : Sekar Suresh, Ealumali Prithiviraj, Nagella Venkatalakshmi, Mohanraj Karthik Ganesh, Lakshmanan Ganesh, Hyun-Jeong Lee, Seppan Prakash

목차

ABSTRACT INTRODUCTION MATERIAL AND METHODS Animals Drug Preparations Sperm Analysis (Sperm Count, Motility, Morphology and Morphometry) Estimation of ROS and LPO Estimation of Enzymic and Non-enzymatic Antioxidant Sperm Analysis (Sperm Count, Motility, Morphology and Morphometry) Single Cell Electrophoresis (Comet Assay) Sperm Chromosomal Integrity Study (Acridine Orange Staining) Statistical Analysis RESULTS Sperm Analysis (Sperm Count, Motility, Morphology and Morphometry) Estimation of ROS and LPO Estimation of Enzymatic and Non-Enzymatic Antioxidant Comet Assay for DNA Damage and Chromatin Integrity DISCUSSION REFERENCES

영어 초록

The study aim is to investigate the free radicals scavenging and spermatogenic potentials, as well as to analyze any reproductive toxicity of ethanolic extract of Mucuna prureins (M. pruriens) Linn. in spermatozoa, under different dosages in normal male rat. Normal rats were randomly selected and suspension of the extract was administered orally at the dosages of 150, 200 and 250 mg/kg body weight of the different groups of male rats (n=6) once in a day for 60 days and grouped as group II, III and IV respectively. Saline treated rats served as control -group I. On the 60th day the animals were sacrificed and the epididymal sperm were subjected to various analyses like level of ROS production, LPO, enzymatic and non enzymatic antioxidant, morphology, morphometry, chromosomal integrity and DNA damage. Results showed significant reduction in ROS production and peroxidation and significant increase in both enzymic and non-enzymic antioxidants in all concentration treated groups when compared with control. Results from all the drug treated groups showed good sperm morphology, increased sperm count and motility. There was no DNA damage and showed normal chromosomal integrity even in 250 mg/kg dose. When compared with control all the three extract treated groups showed increased ROS scavenging activity. However, group II (200 mg/kg) showed significant changes in all the parameters. From the present study it was confirmed that the M. pruriens has potential to improve the sperm qualitatively and quantitatively through scavenging the excess ROS with any adverse side effects. These observations suggest that ethanolic seed extract of M. pruriens may serve as anti-oxidant that can exploit to treat the oxidative stress mediated male factor infertility.

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