Generation of Reactive Oxygen Species and Subsequent DNA Fragmentation in Bovine Cultured Somatic Cells
(주)코리아스칼라
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- 2016.04.01
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- 2011.12
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : In-Sun Hwang, Ho-Jeong Kim, Chun-Keun Park, Boo-Keun Yang, Hee-Tae Cheon
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Culture of Somatic Cells Analysis of ROS Products Comet Assay Statistical Analysis RESULTS ROS Generation Levels in Cultured Somatic Cells DNA Fragmentation of Cultured Somatic Cells DISCUSSIONS REFERENCES
영어 초록
The present study was conducted to examine the reactive oxygen species (ROS) generation levels and subsequent DNA damage in the bovine cultured somatic cells. Bovine ear skin cells were classified by serum starvation, confluence and cycling cells. Cells were stained in 10 μM dichlorohydrofluorescein diacetate (H2DCFDA) or 10 μM hydroxyphenyl fluorescein (HPF) dye to measure the H2O2 or ˙OH radical levels. The samples were examined with a fluorescent microscope, and fluorescence intensity was analyzed in each cell. H2O2 and ˙OH radical levels of cultured somatic cells were high in confluence group (7.1±0.7 and 8.4±0.4 pixels/cell, respectively) and significantly low in serum starvation group (4.9±0.4 and 7.0±0.4 pixels/cell, respectively, p<0.05). Comet tail lengths of serum starvation (148.3±5.7 μm) and confluence (151.1±5.0 μm) groups were found to be significantly (p<0.05) increased in comparison to that of cycling group (137.1±7.5 μm). These results suggest that the culture type of donor cells can affect the ROS generation, which leads the DNA fragmentation of the cells.
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