The viability of B. abortus was markedly decreased in a dosedependent manner in all treatment groups. ... In the cellular cytotoxicity assay, GRE, SC and GS are not cytotoxic at concentrations less than 400 ... In addition, B. abortus intracellular proliferation within macrophages was significantly reduced in cells
pancreatic carcinogenesis via Th2 cells. ... Clostridium difficile infection using long-term frozen stool is effective: clinical efficacy and bacterial viability ... Distrutti E, Monaldi Lr cancer via NKT cells. Science; 360(6391) :eaan5931. 35.
Cell viability and PMI in the co-treated cells were increased, and apoptosis was decreased. ... We evaluated cell viability, plasma membrane integrity (PMI) and apoptotic morphological change in porcine ... In conclusion, taurine and vitamin E can improve cell viability and inhibition of apoptosis in porcine
viability (%) = (Live cell / Live cell+Dead cell) X 100 * 1ml 당 살아있는 세포수 = Live cell / 센 칸 수 x 10 {} ... Isolation하는 동안 cell viability가 낮아지는 것은 mechanical damage 때문이다. ... 또한 pipetting을 제대로 해주지 못해서 세포가 파괴되어서 cell viability가 낮아졌을 가능성도 있다.
Apoptotic effects of CAPE were measured by cell viability assays, Western blotting, 4’-6-diamidino-2- ... The result of cell viability assay showed that CAPE displayed a strong growth-inhibitory effect in a ... in MC-3 cells.
Interestingly, the 3D morphological cells showed 42% higher cell viability than those on the 2D against ... In addition, we conducted a cell viability test in the presence of radioisotopes. ... Using this method, we can perform the 3D cell culture system with three different cell lines such as
The viability of frozen-thawed cell was 60 % for combinated method, and 41 % for general method. ... Increase concentraition of FBS in frozen medium from 20 % to 80 % made viability of thawed cell from ... The viability of thawed cells were 33.9% frozen at 2 embryos/ 0.5ml, and 43.6 % frozen at 20 embryos/
Spermatogonial stem cells (SSCs) developed into sperms through spermatogenesis have been utilized as ... Presently, SSCs can be enriched on testicular stromal cells (TSCs), but there are no systematic researches ... For these, proliferation and viability were measured and compared by culturing ICR outbred or DBA/2 inbred
따라서 100uM 까지는 H2O2가 cell viability에 치명적인 영향을 미치지는 않지만 500uM에서는 치명적임을 유추할 수 있다. ... XTT와 MTT의 차이 XTT와 XTT모두 formazan product로 cell viability를 측정하는 데에 쓰이며 가장 일반적인 방법이 MTT 방법이다. ... 24hr 후 cell이 바닥에 잘 붙었는지 현미경으로 확인한다.
Neither GDF, BMP15 alone nor in combination affects the number and viability of cumulus cells or the ... The treatment of BMP15 increased the cell numbers of both inner cell mass (ICM) and TE cells (P<0.05 ... However, the treatment of GDF9 on porcine oocytes increased the number of trophectodermal (TE) cells
A colorimetric MTS assay was used to examine cell viability. ... In the present study, we focused on its effect on the induction of apoptotic cell death to inhibit cell ... growth and its molecular mechanism in HSC-4 human oral cancer cells.
But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and ... Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change ... cell morphology and proliferation.
viability in temporal basis via MTT analysis on RAW264.7 macrophage cells. ... However, nectar showed the dosedependent cytotoxicity on RAW264.7 macrophage cells after 48 h. ... , we investigated the 70% MeOH extracts (flower, leaf, stem) and nectar of C. grandiflora for their cell
Then, we examined the roles of Hsp90 inhibitor on viability of primary cells in pigs. ... The results showed that viability of pESF cells were decreased by treatment of 17-AAG (2 μM) for 24 hr ... Inhibitory effect of Hsp90 by inducing cell cycle arrest and apoptosis in the cancer cell was reported
실험목표: Mouse를 부검하여 임파 장기들을 관찰하고, Thymus cell의 viability 관찰 및 cell counting을 진행 한다. 3. ... 현미경으로 관찰하는 부분에 미숙한 점이 있어서 live cell과 dead cell의 차이점 정도만 직접 관찰했고, 정확한 cell counting 은 교수님께서 해주셨다. ... 예를 들어, count한 cell의 수가 25개인 경우, sample 1ml안에는 25 x 10⁴개 만큼의 cell이 있다고 계산하게 된다.
viability / toxicity (MTT assay 등)를 한번에 multidetection mode로 측정할 수 있는 장비이다. ... application에 기본적으로 필요한 장비로, 한번에 하나의 sample값을 측정하는 cuvette type photometer와 달리 well plate단위로 다수의 단백질 정량, ELISA, cell
실험결과, 가해준 VPA의 양이 늘어날수록 cell viability가 감소했음을 알 수 있었다. ... cell cycle profile을 감소시키지 않았다고 한다. [4] 우리의 실험결과에서는 VPA 농도 4mM과 8mM에 의해 cell viability가 감소했으므로, cell ... Discussion 이번 실험에서는 제대혈 줄기세포에 서로 다른 양의 VPA를 가해준 후 농도에 따라 cell viability가 어떻게 차이나는지 비교해 보았다.
된다. counting한 것을 토대로 cell viability와 death rate를 측정한다. ... -계산법(calculations)- Cell viability(%) = total viable cells(unstained) / total cells(stained and unstained ... ) * 100 Cell death(%) = total death cells(stained) / total cells(stained and unstained) * 100 3.