항산화제가 Bromopropane에 의해 손상된 돼지 과립막세포의 생존율, 원형질막 온전성 및 apoptosis에 미치는 영향

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 31권 / 3호
ㆍ저자명 : 이승형, 박희우, 이상희, 정희태, 박춘근, 양부근

목차

서 론
재료 및 방법
1. 돼지 과립막세포의 채취 및 1-BP, 2-BP, taurine 및 vitamin E처리
2. 생존율
3. 원형질막 온전성 검사
4. Apoptotic morphological change
5. 통계처리
결과
Bromopropane(1-BP, 2-BP)에 의한 돼지 과립막세포의 손상
Taurine이 돼지 과립막세포의 생존율, 원형질막 온전성 및apoptosis에 미치는 영향
Vitamine E가 돼지 과립막세포의 생존율, 원형질막 온전성 및apoptosis에 미치는 영향
Bromopropane에 의해 손상된 돼지 과립막세포에서 taurine과vitamin E 효과
고찰
REFERENCES

영어 초록

The purpose of this study was to examine the effects of taurine and vitamin E on ovarian granulosa cells damaged by bromopropane (BP) in pigs. We evaluated cell viability, plasma membrane integrity (PMI) and apoptotic morphological change in porcine ovarian granulosa cells. The cells were treated with 1-BP (0, 5.0, 10, and 50 μM), 2-BP (0, 5.0, 10, and 50 mM), taurine (0, 5.0, 10, and 25 mM), and vitamin E (0, 100, 200, and 400 μM) for 24 h. 10 μM 1-BP and 50 μM 2-BP inhibited viability and PMI, and induced apoptosis in porcine ovarian granulosa cells (p < 0.05). Cell viability and PMI were increased by taurine (10 and 25 mM) and vitamin E (100 and 200 μM), and apoptosis decreased (p < 0.05). Finally, the porcine ovarian granulosa cells were co-treated with BPs (10 μM), taurine (10 mM) and/or vitamin E (200 μM). Cell viability and PMI in the co-treated cells were increased, and apoptosis was decreased. In conclusion, taurine and vitamin E can improve cell viability and inhibition of apoptosis in porcine ovarian granulosa cells damaged by bromopropane.

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