Mycelial growth of Lentinula edodes in response to different mixing time, pressure intensity, and substrate porosity

서지정보

ㆍ발행기관 : 한국버섯학회 ㆍ수록지정보 : 한국버섯학회지 / 15권 / 4호
ㆍ저자명 : Hyun You Chang, Geum Hui Seo, Yong Kuk Lee, Sung Woo Jeon

목차

Introduction
Materials and Methods
Results and Discussion
Mycelial growth and density depending on themoisture content of the medium
Mycelial growth and density depending on themixing time of sawdust
Mycelial growth rate and density by sterilizationmethod
Mycelial growth depending on the porosity of thesubstrate
Acknowledgement
References

영어 초록

Biological efficiency (BE), the ratio of fresh mushrooms harvested per dry substrate weight, expressed as the percentage of Lentinula edodes, also known as shiitake, was determined using the ‘Sanjo 701’ strain stored in the Department of Mushroom at the Korea National College of Agriculture and Fisheries. The mycelia were grown in glass columns with varying levels of moisture content and varying mixing periods of 0.5, 1, 2, and 3 hours. The substrate was sterilized using a steam pressure autoclave sterilizer at normal and high pressure to avoid contamination. The results showed that mycelial growth (126 mm/15 days) was optimized at 55% moisture content. The best mycelial growth of 117 mm/15 days was obtained with 2 hours of mixing time. Normal pressure sterilization yielded better results with mycelial growth of 96 mm/15 days at 100°C compared to 88 mm /15 days with sterilization at 121°C. Mycelial density was higher, i.e. 3(+++), with normal pressure sterilization compared to 2(++) with high pressure sterilization. Furthermore, sawdust mixed with 5% woodchips increased the substrate porosity and yielded higher mycelial growth. Thus, we demonstrated that the optimum harvest or potential increased yield of shiitake can be obtained by modulating moisture content, mixing time, and substrate porosity.

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