DNA 메타바코딩을 이용한 광양만 및 어시장 해양 생물 위 내용물 분석

서지정보

ㆍ발행기관 : 한국하천호수학회 ㆍ수록지정보 : 생태와 환경 / 55권 / 4호
ㆍ저자명 : 오건희, 김용준, 김원석, 홍철, 지창우, 곽인실

목차

Abstract
서 론
재료 및 방 법
1. 위 내용물 샘플 확보 및 DNA 메타바코딩
2. 섭식생태 분석
결과 및 고 찰
1. 18S rRNA V9을 이용한 위 내용물 분석
2. 먹이원 정보에 따른 섭식생태 분석
적 요
REFERENCES

영어 초록

Gut contents analysis is essential to predict the impact of organisms on food source changes due to variations of the habitat environment. Previous studies of gut content analysis have been conducted using traditional methods, such as visual observation. However, these studies are limited in analyzing food sources because of the digestive process in gut organ. DNA metabarcoding analysis is a useful method to analyze food sources by supplementing these limitations. We sampled marine fish of Pennahia argentata, Larimichthys polyactis, Crangon affinis, Loligo beka and Sepia officinalis from Gwangyang Bay and Yeosu fisheries market for analyzing gut contents by applying DNA metabarcoding analysis. 18S rRNA v9 primer was used for analyzing food source by DNA metabarcoding. Network and two-way clustering analyses characterized the relationship between organisms and food sources. As a result of comparing metabarcoding of gut contents for P. argentata between sampled from Gwangyang Bay and the fisheries market, fish and Copepoda were analyzed as common food sources. In addition, Decapoda and Copepoda were analyzed as common food sources for L. polyactis and C. affinis, respectively. Copepoda was analyzed as the primary food source for L. beka and S. officinalis. These study results demonstrated that gut contents analysis using DNA metabarcoding reflects diverse and detailed information of biological food sources in the aquatic environment. In addition, it will be possible to provide biological information in the gut to identify key food sources by applying it to the research on the food web in the ecosystem.

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