Polyclonal Antibody to a 37-kDa Recombinant Protein Derived from Bovine 20α-Hydroxysteroid Dehydrogenase
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- 2016.04.01
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- 2012.06
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 2호
ㆍ저자명 : Purevjargal Naidansuren, Kwan-Sik Min
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Materials Tissues Preparation of Bovine 20α-HSD cDNA Cells and Transfections Production of Polyclonal Antibody Bovine CL Cell Culture Western Blot Analysis RESULTS DISCUSSION REFERENCES
영어 초록
We prepared the polyclonal antibody anti-20α-hydroxysteroid dehydrogenase (anti-20α-HSD) against the recombinant full-length protein bovine 20α-HSD in Escherichia coli. The specificity of anti-20α-HSD was demonstrated using Chinese hamster ovary (CHO) cells transfected with recombinant bovine 20α-HSD and bovine placental tissues. According to western blot analysis, anti-20α-HSD specifically recognizes the 37-kDa protein bovine 20α-HSD. The protein is not present in untransfected CHO cells. Anti-20α-HSD also recognizes a specific protein in the ovaries and placenta of other animals. Immunostaining was used to detect expression of bovine 20α-HSD protein in the cultured luteal cells during the estrous cycle later.
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