Variation of Transcribed X-linked Genes in Bovine Embryos Cloned with Fibroblasts at Different Age and Cell Cycle

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 2호
ㆍ저자명 : Byeong-Gyun Jeon, Gyu-Jin Rho

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Media and Chemicals Preparation of Donor Cells Nuclear Transfer and Oocyte Activation In Vitro Culture of Embryos Absolutely Quantitative Analysis of Transcripts by Real Time RT-PCR Relative-Quantitative Telomerase Repeat Amplification Protocol (RQ-TRAP) Experimental Design Statistical Analysis RESULTS Developmental Potential of SCNT Embryos Relative Telomerase Activity (RTA) of SCNT Embryos Transcript Levels of DNMT1 in the Fibroblasts Transcript Levels of X-linked Genes of SCNT Embryos DISCUSSION ACKNOWLEDGEMENTS REFERENCES

영어 초록

The present study compared the developmental potential, telomerase activity and transcript levels of X-linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) in the bovine somatic cell nuclear transfer (SCNT) embryos derived from different age and cell cycle of female donor nucleus. In experiment 1, the fusion rate, cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cells was slightly increased in embryos cloned with fetal fibroblasts compared to those with adult fibroblasts, but there was no significantly (p<0.05) differences. Telomerase activity was also similar in blastocysts cloned with fetal and adult fibroblasts. Up-regulated RPS4X and down-regulated MeCP2, XIAP, and XIST transcript level were observed in blastocysts cloned with adult fibroblasts, compared to those with fetal fibroblasts. In experiment 2, the fusion rate, cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cells was significantly (p<0.05) increased in embryos cloned with fetal fibroblasts at early G1 phase of the cell cycle, compared to those of fetal fibroblasts at late G1 phase. DNMT1 transcript was observed to significantly (p<0.05) increased in the fetal fibroblasts at 3 hrs after trypsin treatment of confluent culture. Further, level of telomerase activity and transcribed X-linked genes was also significantly (p<0.05) higher in the early G1 SCNT blastocysts than those of late G1. The results imply that fetal fibroblasts at early G1 phase induces the enhanced developmental potential and up-regulated telomerase activity and X-linked gene, but aberrant transcript pattern of X-linked genes may be displayed in the SCNT embryos.

참고 자료

없음