Changes of Glycosidase Activity of Frozen-Thawed Spermatozoa in Human
(주)코리아스칼라
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- 2016.04.01
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- 2011.06
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 2호
ㆍ저자명 : Chae-Sik Lee, Sang-Chan Lee, Ji-Eun Lee, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Semen Collection and Preparation Treatment of Spermatozoa for Glycosidase Analyses Measurement of Glycosidase Activity Chlortetracycline (CTC) Assessment of Spermatozoa Spermatozoa Zona-Binding Test Experimental Design RESULTS DISCUSSION ACKNOWLEDGEMENTS REFERENCES
영어 초록
To evaluate the effect of spermatozoa culture on glycosidase activity of frozen-thawed spermatozoa in human, the spermatozoa were treated experimentally and assayed for activities of α-L-fucosidase, α-D-mannosidase, β-D-galactosidase and N-acetyl-β-D-glucosaminidase (β-GlcNAc’ase). The β-GlcNAc’ase activity was at least two-folds higher than other glycosidases regardless of spermatozoa incubation (p<0.05). The spermatozoa motility was decreased with incubation periods, but no effects by different glycosidases on the changes of spermatozoa motility during the various periods of incubation. In all glycosidases, the spermatozoa-zona binding rates in spermatozoa without incubation were higher than in spermatozoa incubated for 2 h (p<0.05). β-GlcNAc’ase is present mainly in the plasma membrane of spermatozoa frozen-thawed in human. It was also shown that the glycosidase activity was increased in all glycosidases in spite of lower sperm-zona binding by spermatozoa incubation.
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