Establishment of Porcine Embryonic Stem Cells by Aggregation of Parthenogenetic Embryos

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 1호
ㆍ저자명 : Jin Yu Zhang, Yun Fei Diao, Reza K. Oqani, Je Sok Wo, Dong Il Jin

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Chemicals In Vitro Maturation Activation of Porcine Oocytes and In Vitro Culture Aggregation of Embryos and In Vitro Culture Preparation of Feeder Cells and Establishment of EmbryonicStem Cells Real-Time Quantitative Polymerase Chain Reaction Statistical Analysis RESULTS Developmental Ability of Aggregated ParthenogeneticEmbryos Total Cell Numbers in Aggregated and Intact Embryos Analysis of Oct-4 Transcription in Aggregated Blastocysts Establishment of Porcine Embryonic Stem Cells DISCUSSION REFERENCES

영어 초록

The pig has been considered to serve as an appropriate model of human disease. Therefore, establishment of porcine embryonic stem cell lines is important. The purpose of the present study was to further work in this direction. We produced porcine parthenogenetic embryos, and separately aggregated two of each of two-cell (2×2), four-cell (2×4), and eight-cell (2×8) embryos derived by parthenogenesis. After culture for 4 days, the developmental ability of the aggregates and total blastocyst cell numbers were evaluated. The percentage of blastocysts was significantly higher in both 2×4- and 2×8-aggregated embryos (58.3±1.9% and 37.2±2.8%, respectively) than in the control or 2×2-aggregated embryos (23.6±1.1% and 12.5±2.4%, respectively). Total blastocyst cell numbers were increased in the 2×4- and 2×8-aggregated embryos (by 44±3.0% and 45±3.3%, respectively) compared with those of control or 2×2-aggregated embryos (30.5±2.1% and 30.7±2.6%, respectively; p<0.05). The levels of mRNA encoding Oct-4 were higher in both the 2×4- and 2×8-aggregated embryos than in the control. When blastocysts derived from 2×4- aggregated embryos or intact normal embryos were cultured on mouse embryonic fibroblast feeder cells to obtain porcine stem cells, blastocysts from aggregated embryos formed colonies that were better in shape compared with those derived from intact blastocysts. Together, the data show that aggregation of porcine embryos not only improves blastocyst quality but also serves as an efficient procedure by which porcine embryonic stem cells can become established.

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