[유전학실험]Restriction enzyme digestion

소개글

"[유전학실험]Restriction enzyme digestion"에 대한 내용입니다.

목차

1. 실험 이론 및 원리
2. 실험 기구 및 시약
3. 실험 방법
4. 주의 사항
5. 실험 결과
6. 토의 사항
7. 참고 문헌

본문내용

1. 실험 이론 및 원리

가. Restriction Enzyme

Restriction enzymes, many of which have been found in bacteria. It recognize and degrade DNA from foreign organisms. Bacteria have confronted the invasion of foreign DNAs for millions of years and have evolved these enzymes as protective mechanisms that preserve their own DNA while destroying the invading DNA.
Each restriction enzyme recognizes only one short sequences, usually 4 to 6 base-pair long. For example, EcoR I, one of the first restriction enzymes to be isolated cuts DNA only at specific sequence.

참고 자료

나노헬릭스, Reliable Partner for DNA Technology, 플라스미드 (Plasmid) DNA 분리, 2015.2.24
Neil A. Campbell 외. 김명원 외 옮김. 「캠벨 생명과학」 10판. 라이프사이언스 출판. 2021. p.246～255