SDS-PAGE 및 Staining(A+보장, 출처가 확실한 양질의 다양한 자료, 검토를 거쳐 더욱 완벽해진 내용)

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"SDS-PAGE 및 Staining(A+보장, 출처가 확실한 양질의 다양한 자료, 검토를 거쳐 더욱 완벽해진 내용)"에 대한 내용입니다.

목차

1. Subject

2. Date

3. Purpose

4. Introduction
1) SDS-PAGE 실험 시 사용되는 재료의 명칭 (Bio-rad 제품)
2) SDS-PAGE의 원리
3) Staining & Destaining 원리
4) 실험에서 사용되는 시약의 조성 및 역할

5. Material & Method
1) SDS-PAGE

6. Result
1) SDS-PAGE
2) Staining & Destaining
3) E.coli streaking, spreading

7. Discussion

8. Reference

9. Assignment
1) E.coli streaking, spreading의 정의 및 목적

본문내용

1. Subject - SDS-PAGE 및 Staining

<중 략>

4. Introduction
1.SDS-PAGE 실험 시 사용되는 재료의 명칭 (Bio-rad 제품)
※ 사용 시 주의 사항 및 자세한 사용법은 ‘10) 실험’ 항목에서 후술.

1) Glass plate : Long & Short plate로 구성. Spacer plate의 경우 볼록하게 튀어나온 면이 있음. Short plate 와 Spacer plate를 결합하여 Polyacrylamide gel을 주조하기위해 사용.
2) Casting frame : 틈에 Glass plate을 끼워 넣어 사용. Glass plate를 고정 시키는 기능.
3) Casting stand : Glass plate이 장착 된 Casting frame을 고정시켜 gel을 굳히는 기능.
4) Casting Stand Gaskets : Casting stand과 함께 사용. 고무재질로 Casting frame의 밑바닥을 밀봉시킴.
5) Comb : gel을 굳힐 때 sample이 들어갈 well을 만드는 기능.

참고 자료

Jeremy M. Berg, 2007, Biochemistry 6th ed, W.H.Freeman&Company
“SDS-PAGE”, wikipedia, 2020.10.01., https://en.wikipedia.org/wiki/SDS-PAGE
“SDS-PAGE”, bio-rad, 2020.10.01., http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf
“SDS-PAGE”, bio-rad, 2020.10.01., https://www.bio-rad.com/ko-kr/applications-technologies/sds-page-analysis?ID=LW7FGX4VY
“SDS-PAGE”, bio-rad, 2020.10.01., https://www.bio-rad.com/ko-kr/product/mini-protean-tetra-handcast-systems?ID=N3F2W9KG4#fragment-3
“SDS-PAGE”, bio-rad, 2020.10.01., https://www.bio-rad.com/ko-kr/category/protein-gels?ID=N3F2J815&WT.mc_id=yt-lsd-ww-miniproteangels-20120827-XnEdmk1Sqvg
Jeremy M. Berg, 2007, Biochemistry 6th ed, W.H.Freeman&Company
“SDS-PAGE”, wikipedia, 2020.10.01., https://en.wikipedia.org/wiki/SDS-PAGE
“SDS-PAGE”, bio-rad, 2020.10.01., http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf
“SDS-PAGE”, bio-rad, 2020.10.01., https://www.bio-rad.com/ko-kr/applications-technologies/sds-page-analysis?ID=LW7FGX4VY
“sodium dodecyl sulfate”, tcichemical, 2020.10.01., https://www.tcichemicals.com/KR/ko/p/D1403
“acrylamide”, sigmaaldrich, 2020.10.01., https://www.sigmaaldrich.com/catalog/product/sigma/a4058?lang=ko&region=KR
“methylene bis acrylamide”, tcichemicals, 2020.10.01., https://www.tcichemicals.com/KR/ko/p/M0506
“SDS-PAGE”, dynebio, 2020.10.01., http://www.dynebio.co.kr/yc/bbs/board.php?bo_table=data1&wr_id=7&page=3
Jeremy M. Berg, 2007, Biochemistry 6th ed, W.H.Freeman&Company
Debmalya Barh , 2018, Omics Technologies and Bio-Engineering, Elsevier, 317-351
남상욱, 2013, 유전공학의 이해 제 2판, 라이프사이언스
“SDS-PAGE”, wikipedia, 2020.10.01., https://en.wikipedia.org/wiki/SDS-PAGE
“SDS-PAGE”, bio-rad, 2020.10.01., http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf
“SDS-PAGE”, bio-rad, 2020.10.01., https://www.bio-rad.com/ko-kr/applications-technologies/sds-page-analysis?ID=LW7FGX4VY
“staining solution”, dogenbio, 2020.10.02., http://www.dogenbio.com/shop/item.php?it_id=1491201734
“staining solution”, dogenbio, 2020.10.02., http://www.dogenbio.com/shop/item.php?it_id=1491201734
“sds page destain principle”, thermofisher, 2020.10.02., https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/protein-gel-stains.html
“SDS-PAGE”, dynebio, 2020.10.01., http://www.dynebio.co.kr/yc/bbs/board.php?bo_table=data1&wr_id=7&page=3
Jeremy M. Berg, 2007, Biochemistry 6th ed, W.H.Freeman&Company
남상욱, 2013, 유전공학의 이해 제 2판, 라이프사이언스
“SDS-PAGE”, wikipedia, 2020.10.01., https://en.wikipedia.org/wiki/SDS-PAGE
“SDS-PAGE”, bio-rad, 2020.10.01., http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf
“5X sample buffer”, bio-d, 2020.10.01., http://www.bio-d.co.kr/kor/prd/prd_view.php?idx=315&c1=79&c2=83
“5X sample buffer”, thermofisher, 2020.10.01., https://www.thermofisher.com/order/catalog/product/39001?SID=srch-srp-39001#/39001?SID=srch-srp-39001
“5X sample buffer”, tech-innovation, 2020.10.01., http://www.tech-innovation.co.kr/?r=home&c=2/14/109
“5x sds running buffer”, thermofisher, 2020.10.01., https://www.thermofisher.com/order/catalog/product/LC2675?SID=srch-srp-LC2675#/LC2675?SID=srch-srp-LC2675
“SDS-PAGE”, dynebio, 2020.10.01., http://www.dynebio.co.kr/yc/bbs/board.php?bo_table=data1&wr_id=7&page=3