RNA extraction(RNA 추출) A+ 레포트

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"RNA extraction(RNA 추출) A+ 레포트"에 대한 내용입니다.

목차

1. Title
2. Name
3. Purpose
4. Materials
5. Methods
6. Result
7. Discussion
8. Reference

본문내용

-Handling and Restraint
① The mouse is placed on the cage lid with the preferred hand.The tail is pulled gently back by the hand.
② The mouse is quickly and firmly picked up by the scruff of the neck behind the ears with thumb and index finger of other hand.
③ The tail is transferred from the preferred hand to between palm and little or ring finger of the other hand, then held firmly.
④ The mouse is restrained.
-Intragastric administration
① First extend the neck
② A straight line isformed between the mouth and stomach.
③ Intragastric injection using 1.0 ml syringe with 22 G X 1.0 infeeding needle is made.

참고 자료

The laboratory mouse , Hans J Hedrich 외 1명, ISBN 978-0-12-336425-8, 2004, pp528~536 (경구투여)
Rodent Handling and Restraint Techniques, JoVE, Cambridge, MA, (2019),
https://www.jove.com/science-education/10221/rodent-handling-and-restraint-techniques
지방, 두산백과 (식생활>식품첨가물), https://terms.naver.com/entry.nhn?docId=1144195&cid=40942&categoryId=32099
Sexing 101(sexing rats and mice), American Fancy Rat & Mouse association, April 2 2019 http://www.afrma.org/sexing101.htm (수컷 암컷 구별법)
Segmentation of the mouse hippocampal formation in magnetic resonance images, Richards K 외 12명, 2011, June 17 https://www.ncbi.nlm.nih.gov/pubmed/21704710
Protocol: Purification of total RNA from Animal tissues, RNeasy® Mini Handbook 4th edition of Qiagen, June 2012, pp37~42, pp64~69