세포학결과레포트-Immunocytochemistry

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세포학결과레포트-Immunocytochemistry
레포트의 모든 형식을 갖추고
레포트점수 최고점을 받은 것입니다.

목차

1. subject
2. object
3. introduction
4. Materials
5. Methods
6. Results
7. Discussion
8. References

본문내용

1. Subject : Immunocytochemistry
2. Object : Immunocytochemistry의 전반적인 과정을 이해하고 Antibody를 이용한 Immunostaining을 통해 microtubule을 관찰할 수 있다.
3. Introduction
1) Immunocytochemistry (ICC)
-Common lab practice which uses antibodies that target specific peptides or protein antigens in the cell via specific epitopes
-In cases where an immunopositive signal is found, ICC allows researchers to determine which sub-cellular compartments are expressing the antigen

2) ICC procedure
Seeding → Fixation → Permeabilisation → Blocking → Antibody binding → Mounting
(1) Fixation
- Fixation is necessary to prevent artifactual diffusion of soluble tissue components, to arrest enzymatic activity, to avoid decomposition of the structure, and to protect the tissue
-All fixation protocols must
① prevent antigen leakage
② keep the antigen in such a form that it can be recognised efficiently by the antibody
③ maintain the cell structure
-Fixative classes : Organic solvents : ethanol, methanol, acetone
: Cross-linking reagents : paraformaldehyde, glutaraldehyde

참고 자료

강영희 외7인 / 아카데미서적 / 세포생물학/ 1992년/ PP 23∼24 , PP 618∼682
맹주선/ 선진문화사/ 식물생리학 / 1991년/ PP 42∼43, PP 48∼49, PP 66∼68
브리태니커 / 동아일보 공동 출판 /브리태니커 8권/ 1993/ pp 130~142