Comparison of serological tests for bovine brucellosis in South Korea
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
ㆍ수록지정보 : 예방수의학회지 / 47권 / 3호
ㆍ저자명 : Ah-Ryeong Joe, Jin Ju Lee, Eun Ji Yum, Yoon-Jeong Seo, So-Ra Sung, Jeong-Soo Choi, Soon-Seek Yoon
ㆍ저자명 : Ah-Ryeong Joe, Jin Ju Lee, Eun Ji Yum, Yoon-Jeong Seo, So-Ra Sung, Jeong-Soo Choi, Soon-Seek Yoon
목차
INTRODUCTIONMATERIALS AND METHODS
Study area and study design
Collection of samples
Serological tests
Bacterial isolation and identification
Statistical analysis
RESULTS
Serological determination
Comparison of correlation in serological tests
DISCUSSION
ACKNOWLEDGEMENTS
영어 초록
Regarding to diagnosis for bovine brucellosis, more than one serological test should be conducted to confirm the infection by Brucella with a reliable result due to various factors including false positive serological reactions. In this study, we compared confirmatory serological tests to determine the appropriate way to detect and confirm the Brucella infection in South Korea. Several serological tests, including serum agglutination test (SAT), indirect (I)- and competitive (C)-ELISA, and fluorescence polarization assay (FPA), for detection of bovine brucellosis were performed with sera from 537 cattle. In addition, comparison of diagnostic efficacy was performed with bacterial isolation represented true positive. Of 537 serum samples, 426 (79.3% of prevalence), 433 (80.6%), 414 (77.1%), and 409 (76.2%) sera were positive for SAT, C-ELISA, I-ELISA, and FPA respectively. Based on the results of serology, the correlation among the serological tests revealed observed agreements of more than 92% with kappa (k) value of more than 0.77. The correlation between serological tests with bacterial isolation appeared observed agreements of between 79.9% and 84.7% with k value of between 0.42 and 0.59. Particularly, FPA recorded almost perfect agreements with C-ELISA and I-ELISA as well as the highest correlation with bacterial isolation. Accordingly, this investigation presented the comparison of correlation and diagnostic efficacy of serological tests for bovine brucellosis in South Korea. We suggest this finding will be a useful data to re-establish the potential serological diagnostic methods that can apply to maintain the low prevalence.참고 자료
없음"예방수의학회지"의 다른 논문
- Vaccination of Brucella abortus recombinant protein Dap..6페이지
- Unveiling the potent antioxidant potential of Tulsi (Oc..7페이지
- Status analysis of domestic trap- neuter-return for str..4페이지
- Increased phagocytosis of macrophages infected with a Δ..5페이지
- Expression of the VP2 protein of canine parvovirus 2b u..6페이지