제주지역 해수, 수족관물, 유통수산물에서 분리된 장염비브리오균의 분자생물학적 특성

서지정보

ㆍ발행기관 : 한국식품위생안전성학회 ㆍ수록지정보 : 한국식품위생안전성학회지 / 38권 / 4호
ㆍ저자명 : 조만재, 강은옥, 허예슬, 고은아

목차

제목없음
Materials and Method
조사대상 및 시료수집
장염비브리오균 증균배양 및 유전자 보유 여부 확인
장염비브리오균 분리배양 및 동정
장염비브리오균 유전자 최종확인
장염비브리오균 정량검사 및 환경요인 측정
PFGE 실험 및 분석
통계분석
Results and Discussion
시료별 장염비브리오균 분리현황
해수의 환경요인과 장염비브리오균 상관관계
독소 유전자 및 종특이성 유전자 확인
Acknowledgement
국문요약
Conflict of interests
ORCID
References

영어 초록

V. parahaemolyticus causes waterborne and foodborne disease such as acute diarrhea. In this study, V. parahaemolyticus isolates from seawater, fish tanks, and distributed fishery products in Jeju were investigated for potential toxin or species-specific genes (tdh, trh, tlh, and toxR) using RT-PCR and their genetic characteristics were analyzed using Pulsed-field gel electrophoresis (PFGE). Overall, V. parahaemolyticus of 90 strains (36.7%), including 33 strains from seawater, 8 strains from fish tanks, and 50 strains from fishery products, were isolated from 245 samples. All V. parahaemolyticus strains did not detect the tdh gene, whereas all strains detected tlh or toxR genes. In addition, trh genes were detected in 3 strains from seawater and 1 strain from fishery products. Monthly quantitative testing of seawater revealed that V. parahaemolyticus was positively correlated with water temperature. The 90 strains of V. parahemolyticus obtained in this study showed by gene homology between types, ranging from 64.0–97.3%. Among these, thirteen types showed 100% homology between genes. These results indicate that continuous monitoring is needed to facilitate food poisoning epidemiological investigations because some isolated V. parahaemolyticus strains harbored toxin genes and V. parahaemolyticus strains isolated from seawater, fish tanks, and distributed fishery products showed genetic similarity.

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