DNA Barcode(DNA 바코드) 레포트(졸업논문)

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DNA barcode와 DNA Metabarcoding에 대한 레포트(졸업논문)입니다.

총 19페이지로, 참고문헌을 꼼꼼하게 기재하여 도움이 되실거라 생각합니다.

목차

제1장 서론

제2장 본론
1. DNA barcode
1) Principle of DNA barcode
2) DNA barcoding in Taxonomy
3) Types of DNA barcode
2. DNA Metabarcoding
1) Principle of DNA Metabarcoding
2) NGS technologies for DNA metabarcoding

제3장 결론

제4장 참고문헌

본문내용

제 1 장 서 론
종의 분류와 식별은 과학자들에게 중요한 과제이다. 지구상에는 약 870만 종의 종이 있다. 동물의 경우 지구상에 있는 종은 777만 종으로 예측되지만 95만3434종만 식별된다[1]. 역사적으로 분류학자들은 형태학적 특징으로 종을 분류하고 식별하였다. 1000~1500만 종의 0.01%이상을 정확하게 분류할 수 있는 분류학자는 거의 없기 때문에 형태학적 종 분류법이 지속되기 위해서는 약 15,000명의 분류학자가 요구된다[2,3]. 또한, 형태학적 종 분류법을 이용하는 것에는 크게 4가지 한계점이 있다. 첫째, 종 식별에 사용되는 형태학적 유연성과 유전적 변동성은 부정확한 식별을 야기할 수 있다. 둘째, 이 분류법은 형태학적으로 불분명한 특징을 간과한다. 셋째, 형태학적 특징은 종종 성별에 대한 특정 성장 단계에만 유용하기 때문에 많은 생물을 식별할 수 없다. 마지막으로 형태학적 특징을 사용하면 잘못 분류, 식별하는 경우가 많아 고도의 전문지식이 요구된다[4]. 그래서 이러한 단점을 극복하면서 종을 분류하는 새로운 방법이 필요하게 되었다. 이 논문은 이러한 필요성에 의해 개발된 새로운 기술인 DNA barcoding의 원리와 활용 및 더 발전된 형태의 기술인 DNA metabarcoding에 대해 살펴보았다.

제 2 장 본 론
1. DNA barcode
1) Principle of DNA barcode
DNA barcoding은 생명체가 가지고 있는 독특한 염기서열을 이용하여 다른 종과의 차이를 나타낼 수 있는 일종의 유전자 식별법(ID)이며, 이러한 종들을 식별하는 데 사용되는 유전 영역을 DNA barcode라고 한다. DNA barcode는 2003년 Paul Hebert 연구팀이 발표한 ‘Biological identifications through DNA barcodes’ 논문에 의해 알려졌다[4]. 그들은 유전체의 일반 영역에서 추출한 DNA 조각을 이용하여 종의 식별과 분류를 가능하게 하는 새로운 시스템을 제안했다.

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