Effect of Alpha Lipoic Acid on in vitro Maturation of Porcine Oocytes and Subsequent Embryonic Development after Parthenogenetic Activationa

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 32권 / 4호
ㆍ저자명 : Young-Hun Kang, Sang-Hwan Hyun

목차

INTRODUCTION
MATERIALS and METHODS
1. Chemicals
2. Oocyte collection and in vitro maturation
3. Measurement of intracellular GSH and ROS levels
4. Parthenogenetic activation of oocytes
5. In vitro embryo culture
6. Embryo evaluation and total cell count of blastocysts
7. Gene expression analysis via quantitative real-time polymerase chain reaction
8. Experimental design
9. Statistical analysis
RESULTS
1. Effect of Alpha lipoic acid on oocyte maturation during IVM
2. Effect of ALA supplemented to IVM media on subsequent embryonic development after PA
3. Effect of ALA treatment during IVM on gene expression in oocytes and cumulus cells
DISCUSSION
ACKNOWLEDGEMENT
REFERENCES

영어 초록

Alpha lipoic acid (ALA) is a biological membranes compound. As the antioxidant, it decreases the oxidized forms of other antioxidant substances such as vitamin C, vitamin E, and glutathione (GSH). To examine the effect of ALA on the in vitro maturation (IVM) of porcine oocytes, we investigated intracellular GSH and reactive oxygen species (ROS) levels, and subsequent embryonic development after parthenogenetic activation (PA). Intracellular GSH levels in oocytes treated with 50uM ALA increased significantly (P < 0.05) and exhibited a significant (P < 0.05) decrease in intracellular ROS levels compared with the control group. Oocytes matured with 50 uM of ALA during IVM displayed significantly higher cleavage rates (67.8% vs. 83.4%, respectively), and higher blastocyst formation rates and total cell number of blastocysts after PA (31.6%, 58.49 vs. 46.8%, 68.58, respectively) than the control group. In conclusion, these results suggest that treatment with ALA during IVM improves the cytoplasmic maturation of porcine oocytes by increasing the intracellular GSH levels, thereby decreasing the intracellular ROS levels and subsequent embryonic developmental potential of PA.

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