Effect of Transfer Temperature of Epididymis on Survival Rate of Semen and B6D2F1 Mice Developmental Capacity
(주)코리아스칼라
- 최초 등록일
- 2023.04.05
- 최종 저작일
- 2016.12
- 8페이지/ 어도비 PDF
- 가격 4,000원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 31권 / 4호
ㆍ저자명 : Dan-Bi Lee, Kee Sang Park, Byoung Boo Seo
영어 초록
This study was conducted to evaluate the effect of transfer temperature of epididymis on survival rate of semen and development ability of B6D2F1 mice embryos. No significant differences were noted in the survival rate of semen (59.0% 0.1 vs. 47.6% 0.1), in vitro fertilization rate (90.7% 0.1 vs. 90.7% 0.1), developmental rate (90.0% 0.1 vs. 90.0% 0.1), and blastocysts formation rate (53.1% 0.2 vs. 52.3% 0.2) between groups. (NS; P>0.05). However, the zona hatched rate was significantly higher in the 4C group compared to those of the 37C group (47.8% 0.1 vs. 25.6% 0.2; p0.05). When it comes to cell numbers of blastocysts, the % ICM (/total cells) was significantly higher in the group of 4C compared to the 37C (27.0% 0.1 vs. 18.3% 0.1; p0.05). However there were no differences in total cell numbers (72.7 31.6 vs. 62.0 36.6), ICM cell numbers (17.0 7.8 vs. 14.6 8.6), TE cell numbers (55.8 29.8 vs. 64.0 24.4), the ratio of ICM:TE (1:4.2 4.1 vs. 1:6.4 7.2) between two groups (NS; P0.05).Taken altogether, it is expected to achieve the best developmental ability of B6D2F1 mice embryos in the transfer temperature of epididymis. Also these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice. In future, therefore, it is expected that results herein might be applied for in vitro culture of human embryos.
참고 자료
없음
"Journal of Embryo Transfer"의 다른 논문
더보기 (1/6)