Improved Cold Tolerance by Transformation with soybean SCOF-1 Gene in Populus alba
(주)코리아스칼라
- 최초 등록일
- 2023.04.05
- 최종 저작일
- 2016.06
- 11페이지/ 어도비 PDF
- 가격 4,200원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 경상대학교 농업생명과학연구원
ㆍ수록지정보 : 농업생명과학연구 / 50권 / 3호
ㆍ저자명 : Yong-Duck Kim
영어 초록
The introduction of stress up-regulated genes into heterologous plants is found to confer stability trait to plants in otherwise hostile environments. The soybean cold inducible factor-1 gene(SCOF-1) encoding a zinc finger transcription factor was introduced into Populus alba L.(white poplar) by co-culture with Agrobacterium tumefaciens GV3101 that carried the binary vector, pGA643/SCOF1. The putative P. alba transformed plants were selected on Murashige and Skoog medium supplemented with 2.22M 6-benzylaminopurine, 1.18mM carbenicillin, and 28.47M hygromycin. The integration of SCOF-1 into the P. alba genome was also established by polymerase chain reaction(PCR), and the expression of the gene was confirmed by reverse transcription PCR. After exposure for 4h at 4 followed by 48h recovery at 27, the transgenic plants completely recovered to normal growth status. The chlorophyll content in transgenic plants was higher than that in wild type plants after chilling treatment. However, the moisture content and total phenolics was also not significantly different in transgenic and wild type plants after cold treatment.
참고 자료
없음
"농업생명과학연구"의 다른 논문
더보기 (5/10)