Increased SOX2 expression in three-dimensional sphere culture of dental pulp stem cells
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 45권 / 4호
ㆍ저자명 : Eun Jin Seo, Il Ho Jang
ㆍ저자명 : Eun Jin Seo, Il Ho Jang
목차
IntroductionMaterials and Methods
1. Cell culture
2. Isolation and culture of primary dental pulp stemcells
3. Organoid culture of primary dental pulp cells
4. Sphere culture of DPSCs and pDPSCs
5. Immunofluorescence staining
6. RNA isolation and quantitative reverse transcriptionpolymerase chain reaction (RT-PCR)
Results
1. Organoid culture of human DPCs
2. Sphere formation of pDPSCs in serum-freesuspension culture
3. Sphere formation of conventional DPSCs
4. pDPSC Spheres and DPSC spheres express SOX2
Discussion
Acknowledgements
Conflicts of Interest
References
영어 초록
Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and hold great potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated from teeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to twodimensional (2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Although the establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stable generation of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, a significant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. These results indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culture may act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics of dental pulp stem cells.참고 자료
없음"International Journal of Oral Biology"의 다른 논문
- Role of microglial activation on neuronal excitability ..7페이지
- Antitumor effects of octyl gallate on hypopharyngeal ca..7페이지
- Induction of anti-aquaporin 5 autoantibodies by molecul..7페이지
- High-salt and glucose diet attenuates alveolar bone rec..7페이지
- Alteration of cellular events in tooth development by c..7페이지
- Green tea polyphenol (–)-epigallocatechin-3-gallate pre..11페이지
- L-ascorbic acid induces apoptosis in human laryngeal ep..10페이지
- High concentration of calcium represses osteoblast diff..7페이지
- Anticancer effects of D-pinitol in human oral squamous ..10페이지
- Inhibitory effects of Coptis chinensis extract on the g..9페이지