DNA metabarcoding을 이용한 이매패류 공식 전후 먹이원 분석

서지정보

ㆍ발행기관 : 한국하천호수학회 ㆍ수록지정보 : 생태와 환경 / 55권 / 4호
ㆍ저자명 : 고봉순, 박재원, 지창우, 곽인실

목차

Abstract
서 론
재료 및 방 법
1. 이매패류 채집 및 DNA 메타바코딩
2. 섭식 생태 분석
결과 및 고 찰
1. DNA metabarcoding을 이용한 이매패류의 위 내용물분석
2. 먹이원 정보를 이용한 이매패류의 섭식 생태 분석
적 요
REFERENCES

영어 초록

Research on food sources through DNA metabarcoding is being used for various organisms based on high resolution and reproducibility. In the study, we investigated the difference in food sources between pre and post-starving in the three bivalve species (Anemina acaeformis, Anodonta woodiana, and Unio douglasiae) through DNA metabarcoding using 18S rRNA V9 primer. The food source of pre-starving appeared in 87 genera, 71 families, 51 orders, 35 classes, and 22 phyla. The primary food sources were the zoo and phytoplankton, including Chlamydomonadales, Euglenales, Ploima, Sphaeropleales, and Stephanodiscales. However, all zoo and phytoplankton were not observed after starving except Schizopyrenida and Rotifera. In Levin’s niche breadth analysis, the Bi index of A. woodiana is 0.3, which was higher than A. acaeformis (0.14) and U. douglasiae (0.21), indicating that they feed on various food sources. The niche overlap of A. acaeformis was measured as 0.78 in A. woodiana, 0.7 in U. douglasiae showing a relative high value compared to other bivalves. The trophic level of A. acaeformis, A. woodiana, and U. douglasiae based on the food source information were investigated as 2.0, 2.0, and 2.5, respectively. The results of the previous study on the trophic level using stable isotopes showed 1.8 to 2.4 values were similar to the results of this study. These results suggest that DNA metabarcoding can be an effective analyzing tool for the gut content in the bivalves.

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