Naringin enhances the migration and osteogenic differentiation of human dental pulp stem cells

서지정보

ㆍ발행기관 : 대한구강생물학회 ㆍ수록지정보 : International Journal of Oral Biology / 47권 / 4호
ㆍ저자명 : Yeon Kim, Hyun-Joo Park, Mi-Kyoung Kim, Yong-Il Kim, Soo-Kyung Bae, Hyung Joon Kim, Moon-Kyoung Bae

목차

Introduction
Materials and Methods
1. 실험 재료
2. 세포 배양
3. 세포 성장률 분석
4. 세포 이동 능력 분석(transwell migration assay)
5. 상처 치유 이동 분석(wound healing migration assay)
6. 실시간 역전사 중합효소연쇄반응(reverse transcriptionquantitativereal-time polymerase chain reaction,RT-PCR)
7. Western immunoblot analysis
8. ALP 염색
9. Alizarin red S (ARS) 염색
10. 통계처리
Results
1. Naringin이 HDPSCs의 이동에 미치는 영향
2. Naringin이 HDPSCs의 CXCR4 및 MMP-2 발현에 미치는영향
3. Naringin이 유도한 HDPSCs의 이동에서 CXCR4 및MMP-2의 관련성
4. Naringin이 HDPSCs의 골분화능에 미치는 영향
Discussion
Acknowledgements
Conflicts of Interest
References

영어 초록

Bioactive flavonoids have been shown to improve the biological activity of stem cells derived from different sources in tissue regeneration. The goal of this study was to see how naringin, a natural flavonoid discovered in citrus fruits, affected the biological properties of human dental pulp stem cells (HDPSCs). In this study, we found that naringin increases the migratory ability of HDPSCs. Naringin increased matrix metalloproteinase-2 (MMP-2) and C-X-C chemokine receptor type 4 (CXCR4) mRNA and protein expression in HDPSCs. ARP100, a selective MMP-2 inhibitor, and AMD3100, a CXCR4 antagonist, both inhibited the naringin-induced migration of HDPSCs. Furthermore, naringin increased osteogenic differentiation of HDPSCs and the expression of the osteogenic-related marker, alkaline phosphatase in HDPSCs. Taken together, our findings suggest that naringin may be beneficial on dental tissue or bone regeneration by increasing the biological activities of HDPSCs.

참고 자료

없음