한국인 모유영양아로부터 분리한 Enterococcus faecium ID9201의 콜레스테롤 저하효과

서지정보

ㆍ발행기관 : 한국유산균학회 ㆍ수록지정보 : Current Topic in Lactic Acid Bacteria and Probiotics / 3권 / 2호
ㆍ저자명 : 이승훈, 김영후, 김민구, 강재훈, 강대중

목차

재료 및 방법
균주의 분리
콜레스테롤 저하균주의 선별
콜레스테롤 흡착균주의 선별
균주의 동정
형태학적 동정
이화학적 동정
분자생물학적 동정
In vivo 혈중 콜레스테롤 저하능
결 과
유산균의 선발
균주의 동정
In vivo 콜레스테롤 저하작용
고 찰
요 약
참고문헌

영어 초록

We selected lactic acid bacteria to reduce cholesterol levels in vitro and in vivo. Among the 140 strains tested, a strain ID9201 was shown to reduce cholesterol in the spent medium over 30% and to increase cholesterol in cell pellet. A strain ID9201 isolated from breast milk-fed Korean infant feces was identified as Enterococcus faecium on the basis of morphological analysis, biochemical analysis (API kit), and 16S rDNA sequence analysis. A freeze-dried powder of the strain was fed to Sprague-Dawley(SD) rats with high cholesterol diet for 6 wk. In the control group fed cholesterol enriched diet without probiotics, the serum cholesterol increased by 1.9 times in comparison with the initial value. Among the test group, ID9201 group showed the lowest cholesterol increase, 1.5 times of initial serum cholesterol, as in the blank group fed normal diet without probiotics. Based on these data, we assumed that ID9201 inhibited intestinal absorption of dietary cholesterol.

참고 자료

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