청국장에서 분리한 BacillussubtilisGS-2에 의한 Poly(γ-GlutamicAcid) 생산의 최적 배양조건

서지정보

ㆍ발행기관 : 한국식품영양학회 ㆍ수록지정보 : 한국식품영양학회지 / 25권 / 3호
ㆍ저자명 : 방병호, 이문수, 김관필, 이동희

한국어 초록

Poly-γ-glutamic Acid(γ-PGA)를 다량 생산하는 균주를 우리나라의 전통발효식품인 청국장으로부터 Bacillus subtilis GS-2를 분리하였다. 이 균은 glutamic acid 의존형 균으로, 이 균에 의한 γ-PGA 생산 최적 조건을 검토한 바, 단순배 지(L-glutamic acid 2.0%, glucose 1.0%, NH<sub>4</sub>Cl 0.5%, KH<sub>2</sub>PO<sub>4</sub> 0.05%, MgSO<sub>4</sub>, 7H<sub>2</sub>O 0.01%, pH 7.0)로 진탕배양(220 rpm) 하였을 때, 배양시간 48시간, 최적온도 33℃, 그리고 초기 pH 6.5로 나타났다. 영양원으로 glutamic acid 3%, sucrose 3%, NH<sub>4</sub>Cl 0.25%, KH<sub>2</sub>PO<sub>4</sub> 0.15%, MgSO<sub>4</sub>, 7H<sub>2</sub>O 0.015%에서 γ-PGA 최대 생산량이 31.0 g/ℓ이었다. Key words: Chungkookjang, culture medium, poly(γ-glutamic acid), PGA, soybean paste

영어 초록

A bacterium strain GS-2 isolated from the Korean traditional seasoning food, Chungkookjang and was determined to produce large amounts of γ-PGA with high productivity when provided with simple nutrients (L-glutamic acid 2.0%, glucose 1.0%, NH<sub>4</sub>Cl 0.5%, KH<sub>2</sub>PO<sub>4</sub> 0.05%, MgSO<sub>4</sub>, 7H<sub>2</sub>O 0.01%, pH 7.0). In this study, the culture medium for this strain was optimized for the production of γ-PGA. The Bacillus subtilis GS-2 required supplementation with L-glutamic acid and other nutrients for maximal production of γ-PGA. The optimal culture conditions for γ-PGA production were a 48 hr culture time, a temperature of 33℃ and initial pH of 6.5 by rotary shaking (220 rpm). A maximum γ-PGA production of 31.0 g/ℓ was obtained with L-glutamic acid (30 g/ℓ), sucrose (the main carbon source, 30 g/ℓ), NH<sub>4</sub>Cl (the main nitrogen source, 2.5 g/ℓ), KH<sub>2</sub>PO<sub>4</sub> (1.5 g/ℓ) and MgSO<sub>4</sub>, 7H<sub>2</sub>O (0.15 g/ℓ) in the culture medium.

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