Proteomic Analysis of the Increased Proteins in Peroxiredoxin Ⅱ Deficient RBCs
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 1호
ㆍ저자명 : Hee-Young Yang, Tae-Hoon Lee
ㆍ저자명 : Hee-Young Yang, Tae-Hoon Lee
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Animals RBC Fractionation The RBC Proteome Nano-UPLC-MSE Tandem Mass Spectrometry Protein Identification and Quantitative Analysis Bioinformatics Analysis Western Blotting RESULTS Identification of Proteins Differentially Regulated in Prdx Ⅱ Deficient RBCs Protein Network Comprised of Identified Proteins RegulatedBy Prdx Ⅱ Deficiency Verification of the Expressional Changes in Prdx Ⅱ—/—RBCs DISCUSSION REFERENCES영어 초록
Peroxiredoxin Ⅱ (Prdx Ⅱ; a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Prdx Ⅱ has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in a variety of disease states associated with oxidative stress, including cancer and aging-associated pathologies. But, the precise mechanism is still obscure in various aspects of aging containing ovarian aging. Identification and relative quantification of the increased proteins affected by Prdx Ⅱ deficiency may help identify novel signaling mechanisms that are important for oxidative stress-related diseases. To identify the increased proteins in Prdx Ⅱ—/— mice, we performed RBC comparative proteome analysis in membrane fraction and cytosolic fractions by nano-UPLC-MSE shotgun proteomics. We found the increased 86 proteins in membrane (32 proteins) and cytosolic (54 proteins) fractions, and analyzed comparative expression pattern in healthy RBCs of Prdx Ⅱ+/+ mice, healthy RBCs of Prdx Ⅱ—/— mice, and abnormal RBCs of Prdx Ⅱ—/— mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cellular morphology and assembly, cell-cell interaction, metabolism, and stress-induced signaling. Moreover, protein networks among the increased proteins were analyzed to associate with various diseases. Taken together, RBC proteome may provide clues to understand the clue about redox-imbalanced diseases.참고 자료
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