Effects of Genotypes on In Vitro Maturation and Fertilization of Frozen-Thawed Porcine Oocytes
(주)코리아스칼라
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- 2016.04.01
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- 2005.12
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 29권 / 4호
ㆍ저자명 : Jia, Y. H., H. J. Jin, M. S. Wee, H. T. Cheong, B. K. Yang, C. K. Park
목차
INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES
영어 초록
In the present study, we investigated the effects of genotypes on in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes. The porcine cumulus-oocyte complexes (COCs) were divided into four groups according to whether they were: (1) in vitro matured; (2) cryopreserved and in vitro matured; (3) in vitro fertilized and (4) cryopreserved, and in vitro fertilized. Maturation of porcine COCs was accomplished by incubation in NCSU23 medium. Immature oocytes were cryopreserved by Open Pulled Straws (OPS) method according to Vajta et al., (1998). Oocytes stained by Acetic-Orcein method were observed under the microscope. DNA extracted from the ovaries was analyzed by RAPD (random amplified polymorphic DNA) and SSCP (single strand conformational polymorphisrrt) method. The rates of oocytes maturation and fertilization were significantly high in AA genotype. The results indicated that in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes may be affected by genotypes in pigs.
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