Feeder Independent Culture of Mouse Embryonic Stem Cells
(주)코리아스칼라
- 최초 등록일
- 2016.04.01
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- 2012.12
- 4페이지/ 어도비 PDF
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 4호
ㆍ저자명 : Myoung Ok Kim, Zae Young Ryoo
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Cell Lines Feeder Preparation and Embryonic Stem (ES) Cell Culture Feeder Free Culture of Embryonic Stem (ES) Cell Induce Differentiation by Withdrawal of LIF RESULTS Phenotype of ES Cell and Feeder The ES Cell Culture on the Feeder Free Condition The ES Cell Induce Differentiation on the Feeder FreeCondition DISCUSSION REFERENCES
영어 초록
Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.
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