LPS로 자극한 microglia BV2 cell에서 Cyrtomium fortunei J.Sm. 추출물의 항염증 효과

서지정보

ㆍ발행기관 : 한국식생활문화학회 ㆍ수록지정보 : 韓國食生活文化學會誌 / 38권 / 3호
ㆍ저자명 : 최지원, 김신태, 최상윤, 최인욱, 허진영

목차

I. 서 론
II. 연구 내용 및 방법
1. 재료 및 방법
2. Cyrtomium fortunei J.Sm. 추출물 (CFJ) 제조
3. 세포배양
4. 세포 생존율 측정
5. Nitric Oxide (NO) 생성 저해 효과
6. Western blot을 통한 단백질 발현
7. RT-PCR을 통한 유전자 발현
8. 통계분석
III. 결과 및 고찰
1. CFJ의 세포 생존률과 NO 생성 억제 효능
2. CFJ의 염증성 매개 인자인 iNOS, COX-2 생성 억제 효능
3. CFJ의 염증성 cytokine 생성 억제 효능
4. CFJ의 MAPKs 활성화 억제 효능 검증
5. CFJ의 산화적 스트레스 억제효능
IV. 요약 및 결론
감사의 글
Conflict of Interest

영어 초록

In this study, we investigated the effect of the extracts of Cyrtomium fortunei J.Sm. (CFJ) on lipopolysaccharide (LPS) induced inflammation in mouse BV-2 microglial cells. Nitric oxide (NO) production and cell viability were measured using the Griess reagent and the (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) assay. Inflammatory cytokines were detected by quantitative polymerase chain reaction (qPCR) in BV-2 microglial cells with and without CFJ extracts. Subsequently, mitogen-activated protein kinases (MAPKs) and antioxidant markers were assessed by western blot analysis. It was found that the CFJ extract significantly decreased the production of pro-inflammatory cytokines (interleukin [IL]-6, tumor necrosis factor [TNF]-, and IL-1) and NO in BV-2 microglial cells that were stimulated with LPS. In addition, the expression levels of the phosphorylation of the MAPK family (p38, c-Jun N-terminal kinases [JNK], and extracellularsignal regulated kinase [ERK]) were reduced by CFJ. Also, treatment with CFJ significantly increased the activities of superoxide dismutase type 1(SOD1) and Catalase in BV-2 microglial cells. Our results indicate that CFJ has a potent suppressive effect on the pro-inflammatory responses of activated BV-2 microglia. Therefore, CFJ has the potential to be an effective treatment for neurodegenerative diseases, as it can inhibit the production of inflammatory mediators in activated BV-2 microglial cells.

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