Correlation between carcass grade and progesterone receptor membrane component 1 in Korean Hanwoo
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서지정보
ㆍ발행기관 : 충북대학교 동물의학연구소
ㆍ수록지정보 : Journal of Biomedical and Translational Research / 21권 / 2호
ㆍ저자명 : Sang Ryeol Lee, Chang Yeon Kim, Young Ho Lee, Jong-ho Kim, Si-yun Ryu, Bae-keun Park, Eui-Ju Hong
ㆍ저자명 : Sang Ryeol Lee, Chang Yeon Kim, Young Ho Lee, Jong-ho Kim, Si-yun Ryu, Bae-keun Park, Eui-Ju Hong
목차
IntroductionMaterials and Methods
Animals
RNA isolation, reverse transcription, and qRT-PCR
Oil Red O staining
Cell culture
siRNA transfection
Statistical analysis
Results
High potential for fatty liver occurrence exists inhigh grade carcasses
Gene levels related to de novo lipogenesis areupregulated in high grade carcasses
Gene levels related to glycolysis are upregulatedin high grade carcasses
Low levels of Pgrmc1 mRNA were observed inlivers from high grade carcasses
PGRMC1 down-regulation was correlated with glycolysisinduction
Discussion
References
영어 초록
Carcass grade primarily depends on marbling of intramuscular fat, which is associated with the texture and tenderness of beef. Accordingly, various economical molecular tests for high intramuscular fat in beef have been attempted. Especially, Hanwoo (Korean Cattle) intramuscular fat has higher levels of monounsaturated fatty acids than that in the beef of other cattle. Intramuscular fats are associated with levels of lipid metabolic genes in the liver transcriptome. Therefore, hepatic triglyceride synthesis can considerably increase intramuscular fat. To investigate the relationship between hepatic lipogenesis and carcass grade, we analyzed 52 Hanwoo liver samples from domestic farms, and evaluated lipid levels and transcript levels of glucose and lipid metabolism-related genes according to carcass grade. Oil-Red-O staining revealed fatty livers in high carcass grades. Moreover, we found significantly higher levels of mRNA for lipogenesis, glycolysis, and triglyceride synthesis genes in high carcass grade livers. Importantly, progesterone receptor membrane component 1 (Pgrmc1) levels were significantly lower in high carcass grade livers. As Pgrmc1 suppression is correlated with induction of de novo lipogenesis (DNL) and glycolysis genes, it has a diagnostic impact for high carcass grades. These results could be used for genetic improvements in carcass grades of cattle. More importantly, as Pgrmc1 can be detected in blood peripheral nucleated cells, it also has value for rapid blood diagnosis.참고 자료
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