Application of a Lateral Flow Immunoassay to Determine Ampicillin Residues in Muscle Tissue of Olive Flounder (Paralichthys olivaceus)
(주)코리아스칼라
- 최초 등록일
- 2023.04.05
- 최종 저작일
- 2013.09
- 4페이지/ 어도비 PDF
- 가격 3,000원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 28권 / 3호
ㆍ저자명 : Chun Nam Cha, Eun-Ah Yu, Min Jung Shin, Eun Kee Park, Hyunju Choi, Suk Kim, Hu Jang Lee
목차
ABSTRACT
Introduction
Materials and Methods
Regents and immunoassay kit
Drug treatment and collection of samples
Analysis of ampicillin residues
Preparation of standard curve
Recovery test
Results
References
영어 초록
Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for the detection of antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 60-min treatment by dipping in water dosed with ampicillin (200-g/ton water), the residue depletion of ampicillin was investigated in 25 cultured olive flounder (Paralichthys olivaceus). Muscles of fish were sampled on the 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of ampicillin in the muscle was determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the muscle residues in olive flounder. To investigate the recovery rate, standard solutions were added to muscle samples to give final concentrations in the muscle of 4 and 8 ng/ml. The recovery rates of all spiked samples were > 96% of the spiked value. Ampicillin was detected in the muscle of fish treated with the drug until the 2nd day of the withdrawal period. The present study showed that the LFIA can be easily adopted to predict ampicillin residues in tissue of farmed fishes.
참고 자료
없음
"한국식품위생안전성학회지"의 다른 논문
더보기 (5/10)