약수터 음용도구의 Bacillus cereus 분포 및 독소 특성
(주)코리아스칼라
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- 2023.04.05
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- 2017.02
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서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 32권 / 1호
ㆍ저자명 : 조아현, 최하나, 허단비, 권순목, 김중범
목차
Materials and Methods
연구대상 및 시료채취
미생물 오염도 측정
독소 유전자 및 독소 단백질 생산 확인실험
항생제 감수성 실험
통계 분석
Results and Discussion
약수 및 음용도구의 미생물 오염도
약수 및 음용도구의 식중독 미생물 오염도
독소 유전자 및 독소 단백질 생산능
항생제 감수성
Acknowledgement
국문요약
References
영어 초록
The purpose of this study was to investigate the microbiological contamination of water and drinking cups in springs and to estimate the toxin gene, enterotoxin production ability and antibiotic susceptibility of foodborne pathogens. Ten spring water and 34 drinking cups were tested. The average number of total aerobic bacteria and coliform bacteria in spring water were 1.8 log CFU/mL and 1.2 log CFU/mL, and in drinking cups were 4.7 log CFU/ 100 cm2 and 1.7 log CFU/100 cm2. Salmonella spp., Staphylococcus aureus, E. coli O157:H7, Listeria monocytogenes, Vibrio parahaemolyticus, Yersinia enterocolitica were not isolated from all of samples but Bacillus cereus was detected in 5 (14.7%) of 34 drinking cups. The nheA and entFM genes were major enterotoxin genes in B. cereus isolated from drinking cups. All of B. cereus tested in this study produce non-heamolytic enterotoxin but only 2 isolates possessed heamolysin BL enterotoxin producing ability. B. cereus was resistant to β-lactam antibiotics. These results revealed that the sanitary conditions of drinking cups in spring should be improved promptly. The substitution carrying a personal drinking cup for the public drinking cups equipped in springs is suggested to prevent food-borne illness.
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